A role for Nrf2 in UVA-mediated heme oxygenase induction and protection from membrane damage in human skin fibroblasts

  • Haibin Li*
  • , Linhao Li
  • , Linhong Deng
  • , Gurinder Singh
  • , Rex M. Tyrrell
  • , J. Li Zhong
  • *Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Our previous study has shown that Ultraviolet-A (UVA) irradiation induces heme oxygenase 1 (HO-1) expression in cultured human primary skin fibroblasts FEK4. In the present study, we demonstrate a coordinated induction of HO-1 and NF-E2-related factor 2 (Nrf2) following UVA irradiation or hemin treatment. The induction of HO-1 by either UVA irradiation or hemin treatment was largely abolished by down-regulation of Nrf2 with its targeted short interfering RNA (siNrf2). The study further reveals that knockdown of Nrf2 protein increased UVA-induced cell death measured by MTS assay. These findings together indicate that Nrf2-mediated induction of HO-1 expression may provide a cytoprotection for human skin cells from oxidative damage.

Original languageEnglish
Title of host publicationOptics in Health Care and Biomedical Optics IV
DOIs
StatePublished - 2010
Externally publishedYes
EventOptics in Health Care and Biomedical Optics IV - Beijing, China
Duration: 18 Oct 201020 Oct 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7845
ISSN (Print)1605-7422

Conference

ConferenceOptics in Health Care and Biomedical Optics IV
Country/TerritoryChina
CityBeijing
Period18/10/1020/10/10

Keywords

  • FEK4
  • hemin
  • HO-1
  • Nrf2
  • UVA

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