A novel light-driven pH-biosensor based on bacteriorhodopsin

Yujia Lv; Nannan Yang; Songhua Li; Shanfu Lu; Yan Xiang

doi:10.1016/j.nanoen.2019.104129

A novel light-driven pH-biosensor based on bacteriorhodopsin

Yujia Lv
, Nannan Yang
, Songhua Li
, Shanfu Lu
, Yan Xiang^*

^*Corresponding author for this work

School of Energy and Power Engineering

Beihang University

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Since it is one of the common proton pump proteins, the bacteriorhodopsin (bR) has attracted significant attention in the field of photoelectronic devices for the development of biohybrid sensors. Here, we report a bR-based photoelectrode for determination of pH in aqueous solutions. Under light irradiation, the bR-based electrode quickly generates a transient photocurrent and follows a reverse photocurrent when the light is switched off. Moreover, the magnitude of forward (I_p) and reverse (I_n) currents are controlled by adjusting the pH of electrolyte. An excellent linear relationship between the I_p/I_n ratio is obtained in the range of pH from 3.5 to 9. In addition, because of the rapid photoelectric response of bR, the bR-based biohybrid sensor is used for the real-time detection of pH in a microfluidic designed system.

Original language	English
Article number	104129
Journal	Nano Energy
Volume	66
DOIs	https://doi.org/10.1016/j.nanoen.2019.104129
State	Published - Dec 2019

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy

Keywords

Photocurrent
Photocycle
Proton pump
pH-biosensor

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10.1016/j.nanoen.2019.104129

Cite this

@article{eea1c3eac87b4daaab715450c22193ec,

title = "A novel light-driven pH-biosensor based on bacteriorhodopsin",

abstract = "Since it is one of the common proton pump proteins, the bacteriorhodopsin (bR) has attracted significant attention in the field of photoelectronic devices for the development of biohybrid sensors. Here, we report a bR-based photoelectrode for determination of pH in aqueous solutions. Under light irradiation, the bR-based electrode quickly generates a transient photocurrent and follows a reverse photocurrent when the light is switched off. Moreover, the magnitude of forward (Ip) and reverse (In) currents are controlled by adjusting the pH of electrolyte. An excellent linear relationship between the Ip/In ratio is obtained in the range of pH from 3.5 to 9. In addition, because of the rapid photoelectric response of bR, the bR-based biohybrid sensor is used for the real-time detection of pH in a microfluidic designed system.",

keywords = "Photocurrent, Photocycle, Proton pump, pH-biosensor",

author = "Yujia Lv and Nannan Yang and Songhua Li and Shanfu Lu and Yan Xiang",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

year = "2019",

month = dec,

doi = "10.1016/j.nanoen.2019.104129",

language = "英语",

volume = "66",

journal = "Nano Energy",

issn = "2211-2855",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - A novel light-driven pH-biosensor based on bacteriorhodopsin

AU - Lv, Yujia

AU - Yang, Nannan

AU - Li, Songhua

AU - Lu, Shanfu

AU - Xiang, Yan

PY - 2019/12

Y1 - 2019/12

N2 - Since it is one of the common proton pump proteins, the bacteriorhodopsin (bR) has attracted significant attention in the field of photoelectronic devices for the development of biohybrid sensors. Here, we report a bR-based photoelectrode for determination of pH in aqueous solutions. Under light irradiation, the bR-based electrode quickly generates a transient photocurrent and follows a reverse photocurrent when the light is switched off. Moreover, the magnitude of forward (Ip) and reverse (In) currents are controlled by adjusting the pH of electrolyte. An excellent linear relationship between the Ip/In ratio is obtained in the range of pH from 3.5 to 9. In addition, because of the rapid photoelectric response of bR, the bR-based biohybrid sensor is used for the real-time detection of pH in a microfluidic designed system.

AB - Since it is one of the common proton pump proteins, the bacteriorhodopsin (bR) has attracted significant attention in the field of photoelectronic devices for the development of biohybrid sensors. Here, we report a bR-based photoelectrode for determination of pH in aqueous solutions. Under light irradiation, the bR-based electrode quickly generates a transient photocurrent and follows a reverse photocurrent when the light is switched off. Moreover, the magnitude of forward (Ip) and reverse (In) currents are controlled by adjusting the pH of electrolyte. An excellent linear relationship between the Ip/In ratio is obtained in the range of pH from 3.5 to 9. In addition, because of the rapid photoelectric response of bR, the bR-based biohybrid sensor is used for the real-time detection of pH in a microfluidic designed system.

KW - Photocurrent

KW - Photocycle

KW - Proton pump

KW - pH-biosensor

UR - https://www.scopus.com/pages/publications/85073027265

U2 - 10.1016/j.nanoen.2019.104129

DO - 10.1016/j.nanoen.2019.104129

M3 - 文章

AN - SCOPUS:85073027265

SN - 2211-2855

VL - 66

JO - Nano Energy

JF - Nano Energy

M1 - 104129

ER -

A novel light-driven pH-biosensor based on bacteriorhodopsin

Abstract

UN SDGs

Keywords

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