TY - JOUR
T1 - A food poisoning caused by ST7 Staphylococcal aureus harboring sea gene in Hainan province, China
AU - Guo, Yahui
AU - Yu, Xiaojie
AU - Wang, Jixiao
AU - Hua, De
AU - You, Yuanhai
AU - Wu, Qingbo
AU - Ji, Qinglong
AU - Zhang, Jianzhong
AU - Li, Liefei
AU - Hu, Yuan
AU - Wu, Zhonghui
AU - Wei, Xiaoyue
AU - Jin, Lianqun
AU - Meng, Fanliang
AU - Yang, Yuhua
AU - Hu, Xiaofeng
AU - Long, Lijin
AU - Hu, Songnian
AU - Qi, Heyuan
AU - Ma, Juncai
AU - Bei, Wenwen
AU - Yan, Xiaomei
AU - Wang, Haibin
AU - He, Zilong
N1 - Publisher Copyright:
Copyright © 2023 Guo, Yu, Wang, Hua, You, Wu, Ji, Zhang, Li, Hu, Wu, Wei, Jin, Meng, Yang, Hu, Long, Hu, Qi, Ma, Bei, Yan, Wang and He.
PY - 2023
Y1 - 2023
N2 - ST7 Staphylococcus aureus is highly prevalent in humans, pigs, as well as food in China; however, staphylococcal food poisoning (SFP) caused by this ST type has rarely been reported. On May 13, 2017, an SFP outbreak caused by ST7 S. aureus strains occurred in two campuses of a kindergarten in Hainan Province, China. We investigated the genomic characteristics and phylogenetic analysis of ST7 SFP strains combined with the 91 ST7 food-borne strains from 12 provinces in China by performing whole-genome sequencing (WGS). There was clear phylogenetic clustering of seven SFP isolates. Six antibiotic genes including blaZ, ANT (4′)-Ib, tetK, lnuA, norA, and lmrS were present in all SFP strains and also showed a higher prevalence rate in 91 food-borne strains. A multiple resistance plasmid pDC53285 was present in SFP strain DC53285. Among 27 enterotoxin genes, only sea and selx were found in all SFP strains. A ФSa3int prophage containing type A immune evasion cluster (sea, scn, sak, and chp) was identified in SFP strain. In conclusion, we concluded that this SFP event was caused by the contamination of cakes with ST7 S. aureus. This study indicated the potential risk of new emergencing ST7 clone for SFP.
AB - ST7 Staphylococcus aureus is highly prevalent in humans, pigs, as well as food in China; however, staphylococcal food poisoning (SFP) caused by this ST type has rarely been reported. On May 13, 2017, an SFP outbreak caused by ST7 S. aureus strains occurred in two campuses of a kindergarten in Hainan Province, China. We investigated the genomic characteristics and phylogenetic analysis of ST7 SFP strains combined with the 91 ST7 food-borne strains from 12 provinces in China by performing whole-genome sequencing (WGS). There was clear phylogenetic clustering of seven SFP isolates. Six antibiotic genes including blaZ, ANT (4′)-Ib, tetK, lnuA, norA, and lmrS were present in all SFP strains and also showed a higher prevalence rate in 91 food-borne strains. A multiple resistance plasmid pDC53285 was present in SFP strain DC53285. Among 27 enterotoxin genes, only sea and selx were found in all SFP strains. A ФSa3int prophage containing type A immune evasion cluster (sea, scn, sak, and chp) was identified in SFP strain. In conclusion, we concluded that this SFP event was caused by the contamination of cakes with ST7 S. aureus. This study indicated the potential risk of new emergencing ST7 clone for SFP.
KW - enterotoxin A
KW - plasmids
KW - prophage
KW - sequence type 7
KW - staphylococcus food poisoning
KW - whole-genome sequencing
UR - https://www.scopus.com/pages/publications/85151754236
U2 - 10.3389/fmicb.2023.1110720
DO - 10.3389/fmicb.2023.1110720
M3 - 文章
AN - SCOPUS:85151754236
SN - 1664-302X
VL - 14
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
M1 - 1110720
ER -