A FINGER-DRIVEN DISPOSABLE MICRO-PLATFORM BASED ON ISOTHERMAL AMPLIFICATION FOR THE APPLICATION OF MULTIPLEXED AND POINT-OF-CARE DIAGNOSIS OF TUBERCULOSIS

In this work, we introduced a portable finger-driven microfluidic chip (Fd-MC) based on recombinase polymerase amplification (RPA) for rapid on-site detection of Tuberculosis. The pre-packaged buffer was driven by the pressure generated by finger-actuated operation to accomplish sequential processes of diagnosis. In-situ fluorescence technique based on FAM probe was implemented for on-chip results read-out. Hence, the Fd-MC can avoid the risk of infection or environmental contamination, and eliminates the need on external pumps and skilled personnel, holding promise to POC testing in the resource-limited area.