@inproceedings{2a284643845f413da3ce408dc08e6701,
title = "A Femtosecond Stimulated Raman Loss (fSRL) microscope for highly sensitive bond-selective imaging",
abstract = "We demonstrate nonlinear vibrational imaging of isolated Raman bands by detecting femtosecond pulse stimulated Raman loss. Femtosecond pulse excitation produces a stimulated Raman loss signal that is 12 times larger than what picosecond pulse excitation produces. The strong signal allowed real-time, bond-selective imaging of deuterated palmitic acid-d31 inside live cells, and 3D sectioning of fat storage in live C. elegans. With the high peak power provided by femtosecond pulses, this system is highly compatible with other nonlinear optical modalities such as two-photon excited fluorescence. With most of the excitation power contributed by the Stokes beam in the 1.0 - 1.2 μm wavelength range, photodamage of biological samples was not observed.",
keywords = "Coherent Raman scattering, Femtosecond laser, Lipid, Multimodal imaging, Nonlinear microscopy, Stimulated Raman scattering (SRS)",
author = "Delong Zhang and Slipchenko, \{Mikhail N.\} and Shuhua Yue and Junjie Li and Cheng, \{Ji Xin\}",
year = "2011",
doi = "10.1117/12.875886",
language = "英语",
isbn = "9780819484406",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Multiphoton Microscopy in the Biomedical Sciences XI",
note = "Multiphoton Microscopy in the Biomedical Sciences XI ; Conference date: 23-01-2011 Through 25-01-2011",
}