A 3D location method of bioluminescence light source based on multi-view projection surface reconstruction

In bioluminescent tomography imaging (BLT), dual-modality fusion (optical modality and structural modality) can make full use of high accuracy 3D geometrical structures provided by structural modality reconstruct 3D surface light flux distribution and bioluminescence inner light source reconstruction. However, compared with the all-optical modality, dual-modality fusion has the problems of complicated fusion system, high cost compared with all-optical system, multifarious and exhaustive date processing, and ionizing radiation (for example, CT). Therefore, the 3D location method of bioluminescence light source based on pure optical 3D geometrical structures has significance for BLT. In this paper, we present a 3D location method of bioluminescence light source based on multi-view projection surface reconstruction, and an all-optical bioluminescence tomography system (AOBTS) is developed for this method. The method consists of 3D surface reconstruction based on multi-view optical projection, multi-view luminescent seamless integration, calibration and quantification of the surface light flux and internal bioluminescence reconstruction. An in-vivo BALB/C mouse with an implanted luminescent light source are used to evaluate the performance of the new method. Compared with the conventional optical methods, the new method improves not only the 3D surface reconstruction method but also the multi-view luminescent seamless integration. It has realized 3D real mouse bioluminescence light source localization, and the preliminary test proves its potential application in clinical trial.