高脂饮食对大鼠主动脉内皮细胞形态及刚度的影响

Objective: To investigate the high-fat diet effect on morphology and stiffness of endothelial cells. Methods: SD rats were randomly divided into high-fat diet group (AS group, n=3) and control group (CON group, n=3). Rat aortic endothelial cells were obtained from rat thoracic aorta by explant method. Cell morphology was observed under inverted microscopy. The mean fluorescent intensity of F-actin in two groups was calculated by immunofluorescence staining. Cell stiffness was measured by atomic force microscopy (AFM). Results: The endothelial cells migrated from tissue plant on the 7th day and formed confluence after cultivation for 14 days. Endothelial cells were identified by factor Ⅷ immunofluorescence staining. Cells in AS group showed enhanced perimeter (P<0.01), aspect ratio (P<0.01), and area (P>0.05), while less circularity (P<0.01) compared with the cells in control group. The mean fluorescence intensity of F-actin in AS group was significantly higher than that in control group (P<0.01). AFM showed that the cell stiffness of AS group was significantly higher than that of control group (P<0.01). Conclusions: High-fat diet would change the morphology and stiffness of endothelial cells, which might subsequently affect their normal function and become an important incentive to AS.