溴化乙锭诱导的短暂脱髓鞘增强PRV的逆行转导效率

Objective: To improve the efficiency of PRV transduction by ethidium bromide (EB) -induced transient demyelination.Methods: 18 adult Wistar rats were randomly divided into muscle group, NS group and EB group(n=6).In the muscle group, 2μl PRV with a titer of 2×10 ⁹ was injected into the tibial anterior muscle and gastrocnemius muscle. NS group was injected with 2μl normal saline (NS) to the sciatic nerve, and 2μl PRV with a titer of 2×10 ⁹ was injected to the same site one week later. In the EB group, 2μl 0.1% EB was injected into the sciatic nerve, and 2μl PRV with a titer of 2×10 ⁹ was injected into the same position one week later. Five days later, perfusion samples were taken and frozen sections were made to observe the infection of neurons at all levels. Methods: A large number of L4-L5 spinal anterior horn neurons, dorsal root ganglion (DRG)neurons, T8 spinal intermediate neurons, C4 spinal intermediate neurons, medulla oblongata, midbrain and cerebral cortex of rats in EB group were labeled by PRV.A small number of neurons at all levels were labeled by PRV in the muscle group and the NS group.Conclusion: EB- induced sciatic nerve demyelination can enhance the reverse transduction efficiency of PRV.