兔激素性股骨头坏死的造模改进及结果评价

The establishment of animal model of femoral head necrosis is of great significance for the clinical treatment of this disease. To successfully induce femoral head necrosis while reducing the mortality of modeling animals, this study improves the commonly used modeling method of steroid-induced femoral head necrosis in rabbits. Ninety male New Zealand white rabbits (2.8～3.2 kg) are randomly divided into control group, conventional model group and improved model group. The control group is only injected with normal saline; the conventional model group is injected with lipopolysaccharide and methylprednisolone; the improved model group is supplemented with penicillin, ranitidine, diclazuril, intestinal lubricant and lactasin based on conventional model. The results show that the death rate of the improved model group (13.3%) is much lower than that of the conventional model group (70%). The Micro-CT and histological results exhibit that the bone volume, bone volume/tissue volume ratio, bone mineral density and trabecular thickness in improved model group are significantly lower than those in control group, while the number of empty bone lacunae is significantly higher than that in control group. These results demonstrate the successful establishment of steroid-induced femoral head necrosis in rabbits.